荧光照明光的单独光束路径
该系统的症结在于，您向系统引入的激发光（能量）越多，光学元件上出现的反射就越多，从而导致"noise" and in "bright" instead of "deep dark"背景。这使得荧光阳性和阴性细胞之间的区分非常复杂。防止这种情况的最佳解决方案"noise"为了避免激发光与观察光束路径的光学元件接触。
The triple beam principle does exactly that; it consists of two parallel beam paths for stereo observation and a separate third beam path exclusively for fluorescence illumination light. The benefit of this principle is that the observation beam paths are free from any direct excitation light. Compared with systems using the same beam paths for observation and illumination, e.g. systems using a fluorescence filter cube with a dichroic filter to separate excitation and emission light, the triple beam principle has a significantly better 信噪比.
明亮的荧光信号
而且，所有三个光束路径的变焦都是同步的。照明光仅在立体显微镜的视场上聚集。通过增加放大倍率，激发光被捆绑得更多，并且单位面积的激发光（能量）数量增加。
另一个优点是，三个光路被设计为彼此非常接近。三个所需的荧光滤光片（一个用于激发，两个用于发射）安装在一个小的，易于更换的滑块中。由于其体积小，在三光束原理立体显微镜设置中最多可以使用四个滤光片。
概述：
1. 光导联轴器
2. 光（全光谱）
3. Excitation filter
4. Excitation light
5. 缩放系统，包括2个观察和1个发射光束路径
6. 共同的主要目标
7. 荧光标记探针
8. Emission light
9. Emission filters
10. Trinoc双目
11.目镜
12.相机
总而言之，三光束原理是一个非常有效的系统 for illuminating specimens with excitation light and observing the resulting fluorescence with a perfect 信噪比. The result is a 亮 fluorescence signal surrounded by a 深深的黑暗 background.
